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The vasodilation in response to serotonin (0.1 μM) was significantly inhibited by free oxyHb and RBCs with 50% or 90% hematocrit (corresponding to 3 and 5.6 mM overall Hb, respectively). Fifty percent hematocrit (3.1 mM Hb) had less inhibition to serotonin-induced dilation than 10 μM free oxyHb. n = 5, basal diameter = 76 ± 2 μm, maximum diameter = 98 ± 3 μm; ∗, P < 0.05, versus control; +, P < 0.05, versus 50% hematocrit.
