Table 1.

Induction methods for RNAi by feeding

	Non-Ind		Ind (1)		Ind (2)		Ind (3)		Ind (4)
Test gene	n	% Phe	n	% Phe	n	% Phe	n	% Phe	n	% Phe
gpb-1	546	0	530	100	309	84	442	97	346	0
unc-22	422	0	255	99	179	80	ND	ND	ND	ND
par-1	ND	ND	313	100	263	100	ND	ND	ND	ND
par-3	ND	ND	391	96	325	11	ND	ND	ND	ND

Four different methods were compared to determine optimal induction conditions for RNAi; non-induced (Non-Ind) bacteria were also included for comparison. Induction conditions (Ind) were as follows: (1) Bacteria were induced on plates with IPTG at room temperature overnight; (2) bacteria were induced in culture at 37°C for 2 h; (3) bacteria were induced on plates with IPTG at 37°C overnight; (4) bacteria were induced in culture at 37°C overnight (see the Materials and methods section for detailed protocols). gpb-1, par-1 and par-3 were scored for percentage of dead embryos, unc-22 was scored for percentage of worms with an uncoordinated phenotype. Data shown represent the progeny of three fed worms. ND, not done; n is the number of worms or embryos scored; %Phe, percentage of worms or embryos with phenotype.