Table 3.
Strength of RNAi by feeding versus injection
Feeding | Injection | |||
Test gene | n | % Phe | n | % Phe |
gpb-1 | 506 | 100 | 175 | 100 |
par-1 | 262 | 100 | 293 | 100 |
par-2 | 257 | 100 | 288 | 100 |
par-3 | 241 | 97 | 540 | 100 |
par-6 | 361 | 100 | 348 | 100 |
cyk-1 | 308 | 55 | 275 | 100 |
skn-1 | 355 | 100 | 311 | 100 |
dnc-1 | 343 | 100 | 309 | 100 |
bir-1 | 391 | 100 | 403 | 100 |
pal-1 | 393 | 100 | 396 | 100 |
dif-1 | 391 | 100 | 244 | 100 |
plk-1 | 402 | 100 | 239 | 100 |
dhc-1 | 269 | 100 | 173 | 100 |
mex-3 | 200 | 100 | 256 | 100 |
Fourteen known maternal-effect embryonic lethal genes were tested to determine the efficiency of RNAi by feeding relative to injection for producing embryonic lethality. Data shown represent the progeny of three fed worms. n, number of embryos scored; %Phe, percentage of embryos with embryonic lethality.