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. 2007 Jan 1;21(1):71–84. doi: 10.1101/gad.1500707

Figure 3.

Figure 3.

miR-133 and miR-1/206 expression is restricted to muscle-derived cell lines. Size-fractionated samples of total RNA from tissue culture cells were probed for the presence of microRNAs. Note that the probe for miR-133 cannot distinguish between miR-133a and miR-133b, and the probe for miR-1/206 cannot distinguish between miR-1 and miR-206. miR-16 is a widely expressed microRNA detected in most cell lines. 5S rRNA was stained with ethidium bromide to normalize for loading. (A) Small RNA Northern probed for miR-133 expression in cell lines. The lane marked “C2C12” contains RNA from proliferating cells, whereas “C2C12 Diff” is RNA from cells treated for 4 d in differentiation medium. Similarly, “P19” is RNA from undifferentiated cells and “P19 Diff” is RNA from retinoic acid-treated P19 cells. (B) Small RNA Northern blot of RNA from differentiating C2C12 cells probed for miR-133 and miR-1/206. Differentiation medium was added at time 0 and RNA was harvested every 48 h through day 10. Note that the miR-1/206 and miR-16 panels are probings of the same blot and are normalized to the same 5S RNA panel.