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. 2000 Nov 21;97(25):13518–13522. doi: 10.1073/pnas.250473497

Figure 2.

Figure 2

Relaxation kinetics of En-HD. (A) Kinetic trace at 25°C and 2.6 M urea on a 24,000-V electrical discharge with a 50-nF capacitor (5° jump). Six traces were averaged (En-HD concentration was 49 μM). Fitting of the observed trace to a single exponential equation gives a relaxation rate of 10,300 ± 200 s−1. (B) Reaction trace at 57°C and 0 M urea on a 30,000-V electrical discharge from a 10-nF capacitor (1° jump). Seventeen traces were averaged (En-HD concentration was 300 μM). Fitting of the observed trace to a single exponential equation gives a relaxation rate of 145,000 ± 500 s−1.