. 2006 Feb 6;147(8):897–904. doi: 10.1038/sj.bjp.0706679

Table 2.

Efficacy (E_max) and sensibility (pD₂) to ACh of the gracilis artery isolated from 3, 12 and 20 m/o WT and DL mice

Conditions	Age
	3 m/o WT		12 m/o WT		20 m/o WT		3 m/o DL		12 m/o DL		20 m/o DL
	E_max	pD₂	E_max	pD₂	E_max	pD₂	E_max	pD₂	E_max	pD₂	E_max	pD₂
40 mM KCL-PSS	48±4^* (6)	7.7±0.1	30±5^*^† (6)	7.9±0.2^*	26±2^*^† (5)	5.2±0.1^*^†^‡	46±4^* (6)	7.6±0.3	30±5^† (8)	7.8±0.1^*	24±5^* (4)	6.9±0.5
INDO+L-NNA	86±3 (7)	7.4±0.2	66±8^† (6)	6.9±0.2	48±4^†^‡ (5)	5.7±0.1^†^‡	95±2^¶ (7)	7.4±0.1	57±8^† (8)	6.3±0.1^†	26±2^†^‡^¶ (5)	6.3±0.1^†
+17-ODYA	89±5 (4)	7.6±0.4	43±6^*^† (7)	7.2±0.2	33±2^*^†^‡ (3)	5.4±0.2^†^‡	42±11^*^¶ (6)	7.2±0.3	57±9 (6)	5.7±0.2^*^†	23±3^†^‡^¶ (5)	5.4±0.5^†^‡
+Ouabain	19±7^* (4)	6.2±0.6^*	22±5^* (6)	7.2±0.2	10±1^*^†^‡ (3)	6.3±0.3^‡	75±10^¶ (7)	6.9±0.2	26±6^*^† (4)	5.9±1.0	2±1^*^†^‡^¶ (7)	NM
+Apamin+Chtx	22±4^* (7)	6.8±0.2	46±2^*^† (6)	7.2±0.1	12±3^*^‡ (3)	NM	25±5^* (5)	6.0±0.4^*	8±2^*^†^‡ (4)	NM	2±1^*^†^‡^¶ (5)	NM
+NAC	NT	NT	66±10 (6)	7.0±0.3	42±8 (3)	5.4±0.1	88±5 (3)	8.1±0.5	84±3^*^¶ (6)	7.1±0.2^*	27±5^‡^¶ (4)	6.4±0.3

Data are expressed as mean±s.e.m. Vessels were preconstricted by 40 mM KCl-PSS or by PE in the presence of INDO (10 μM) and L-NNA (100 μM) combined with apamin and charbdotoxin, 17-ODYA, ouabain or NAC.

P<0.05 compared to INDO+L-NNA.

^†

P<0.05 compared to 3 m/o.

^‡

P<0.05 compared to 12 m/o.

^¶

P<0.05 compared to WT.

NT=not tested.

NM=not measurable.