Figure 4.

Phagocytic behaviour of leucocytes from ANXA1^−/− mice. (a) Phagocytosis of untreated (light shading) or opsonised (dark shading), zymosan by peritoneal macrophages from ANXA1^+/+, ANXA1^+/− and ANXA1^−/− mice. Pooled data from two experiments were analysed relative to ANXA1^+/+ controls, using two-tailed Student's t-test; n=6 per group. ^**P<0.01; ^***P<0.001, significantly different from Anx-1^+/+ opsonised values. ^††P<0.01 different from ANXA1^+/+ unopsonised values. (b) Inhibition by 10 μM hydrocortisone of IgG phagocytosis in peritoneal macrophages from different genotypes, using the RedOxyburst technique. Each experiment is typical of four similar experiments. White columns, % inhibition of reaction maximum; shaded columns, % inhibition of reaction rate. ^*P<0.05; relative to vehicle-treated controls. Inset: Typical trace showing inhibition of reaction by 10 μM hydrocortisone. Each point on the kinetic reaction plot above is the mean of three readings and the error bars represent standard errors of the mean. Reprinted from Hannon et al. (2003) with permission.