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gp160 palmitoylation is critical for viral infectivity. Luciferase-expressing viruses carrying Env mutants were used to infect HOS T4 (CD4+) cells. The infectivity of the virions was measured with a luciferase assay of the infected cell lysates at 48 h postinfection. Raw luciferase data then were normalized to the concentration of the input virus as measured by p24 ELISA (see text). Error bars represent the standard deviation of triplicate experiments.
