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. 2000 Nov 28;97(25):13537–13542. doi: 10.1073/pnas.240460997

Figure 3.

Figure 3

The demonstration of molecular break light specificity and general proof of principle. The observed change in fluorescence intensity over time of an assay containing 3.2 nM break light at 37°C. (a) Break light A with 100 units of BamHI (□), break light B with 100 units of BamHI (○), and break light B without enzyme (●) (10 mM Tris⋅HCl/50 mM NaCl/10 mM MgCl2/1 mM DTT, pH 7.9; λEx = 485 nm, λEm = 517 nM). (b) Break light A with and 10 units of DNaseI (□), break light B with 10 units of DNaseI (○), and break light A without enzyme (●) (40 mM Tris⋅HCl/10 mM MgSO4/1 mM CaCl2, pH 8.0; λEx = 485 nm, λEm = 517 nM).