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. 2000 Nov 28;97(25):13537–13542. doi: 10.1073/pnas.240460997

Figure 4.

Figure 4

The determination of BamHI steady-state kinetic parameters using break light B. (a) The observed change in fluorescence intensity over time of an assay containing a constant 3.2 nM break light B at 37°C (6 mM Tris⋅HCl/100 mM NaCl/6 mM MgCl2/1 mM DTT, pH 7.5; λEx = 485 nm, λEm = 517 nM), BamHI (10 units), and varying nonlabeled substrate oligonucleotide. Total substrate concentrations (including break light): 389 nM (○), 196 nM (□), 81 nM (⋄), 42 nM (▵), 11 nM (●), 7.5 nM (■), and 3.4 nM (⧫). (b) Lineweaver–Burke plot from a after correction for the carrier dilution effect.