Figure 2.

Intracellular cAMP levels in the parental and taxol resistant cells before and after treatment with taxol. cAMP levels were determined using an acetylated version of a cAMP measurement kit from Biomol Research Laboratories. A standard curve of known concentrations of acetylated cAMP was generated for each experiment. Untreated and taxol treated (16 hr) cells were processed as described (see Materials and Methods section). The cAMP level in the taxol resistant cells was compared with those observed in the 2008 cells (5.6 ± 1.1 pmol/mg protein; mean of 5 experiments each performed in duplicate) considered as 1.