Skip to main content
. 2007 Jan 3;104(2):606–611. doi: 10.1073/pnas.0605957104

Fig. 2.

Fig. 2.

Functionally conjoined lymphatic and blood vasculature in the small intestines of Fiaf−/− mice. (A and B) Bright-field immunohistochemistry showing Lyve-1 (red) in hematoxylin-stained sections of small intestine from P7 Fiaf+/+ (A) and Fiaf−/− (B) littermates. Cross-sections of villi from Fiaf+/+ and Fiaf−/− small intestine are displayed in A Right and B Right, respectively. The dilated lymphatic vasculature in the villus core mesenchyme of the Fiaf-deficient mouse is lined by Lyve-1-positive endothelial cells (e.g., arrow) and filled with blood cells (asterisks). (C–H) Immunostaining for Lyve-1 (red) in the small intestines of P7 Fiaf+/+ and Fiaf−/− mice that received an i.v. injection of FITC-labeled BS-1 (blood and lymphatic vascular endothelial cell-binding lectin; green) 30 min before they were killed. Note the presence of dilated FITC-BS-1/Lyve-1-positive lymphatic vessels (orange) at the base of Fiaf−/− villi. Dashed lines in E and H outline the margins of villi. (Scale bars: 50 μm in A and B and 100 μm in C–H.)