Fig. 3.

Development of small intestinal lymphatics in wild-type and Fiaf−/− mice. (A–D) Close approximation of anatomically distinct Lyve-1-positive lymphovasculature (L) and blood vasculature (b) in E18 and P0 wild-type (A and C) and Fiaf−/− (B and D) littermates. At these stages of development, no erythrocytes are seen in the lymphovasculature of knockout (or wild-type) littermates. (E and F) By P3, blood cells are present within the lymphatic vasculature (L*), but not in the extravascular space, of Fiaf−/− animals (compare Insets). (Scale bars: 50 μm in A, B, E, and F and 25 μm in C and D.)