Fig. 5.

Circulating Fiaf rescues the conjoined lymphatico-venous abnormality in Fiaf−/− isografts. Small intestinal isografts were harvested from E15 Fiaf+/+ or Fiaf−/− donors and implanted for 14 days into the dorsal s.c. tissue of adult Fiaf+/+ recipients. Serial sections of harvested isografts were stained with antibodies to Lyve-1 (red) or Prox1 (yellow) and counterstained with hematoxylin. Representative nascent crypt villus units are shown. Lymphovascular development is indistinguishable in wild-type (A) and Fiaf−/− (B) isografts. Black-bordered insets show enlarged views of the black boxed areas: note the absence of dilated, blood-filled lymphatic vessels in the Fiaf−/− isograft. White-bordered insets show Lyve-1-positive lymphatic endothelium with Prox1-positive nuclei (arrows). (Scale bars: 25 μm.)