Table 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pME1901 | aro7∷hisG–URA3–hisG cassette for deletion of ARO7 ORF | This study |
| pME1902 | trp2∷hisG–URA3–hisG cassette for deletion of TRP2 ORF | This study |
| pME1187 | 2-kb EcoRI fragment containing ARO7 in pGEM7(+) | This study |
| pME606 | 2-kb EcoRI fragment containing ARO7c in pJDB207 | Ref. 8 |
| pME1903 | 2.3-kb XbaI/BamHI fragment containing TRP2 in pGEM7(+) | This study |
| pME1904 | 2.3-kb XbaI/BamHI fragment containing TRP2fbr in pGEM7(+) | This study |
| pME1905 | 3-kb AatII/EcoRI fragment containing 5′-ARO7∷pTRP2∷ARO7 replacement cassette in pGEM7(+) | This study |
| pME1906 | 3-kb AatII/EcoRI fragment containing 5′-ARO7∷pTRP2∷ARO7c replacement cassette in pUC19 | This study |
| pME1907 | 3-kb AatII/BamHI fragment containing 5′-TRP2∷pARO7∷TRP2 replacement cassette in pGEM7(+) | This study |
| pME1908 | 3-kb AatII/BamHI fragment containing 5′-TRP2∷pARO7∷TRP2fbr replacement cassette in pUC19 | This study |
| p164 | 2.8-kb SalI/EcoRI fragment containing GCN4 in YCp50 | Ref. 22 |
| p238 | 2.8-kb SalI/EcoRI fragment containing GCN4 with all four upstream ORFs mutated in YCp50 | Ref. 22 |
| pME1909 | 470-bp SspI/EcoRV fragment of ARO7 ORF and 465-bp Eco72I/EcoRV fragment of TRP2 ORF in pBluescript II KS | This study |