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RT-PCR analysis of epiC genes during a time course of colonization of tomato by P. infestans. Total RNA isolated from noninfected leaves (To), infected leaves of tomato, 1, 2, 3, or 4 d after inoculation with P. infestans, and from P. infestans mycelium grown in synthetic medium (My) was used in RT-PCR amplifications. Amplifications of P. infestans ef2α were used as constitutive controls to determine the relative expression of epiC genes.
