Figure 6.

Lipid and fatty acid composition of DRMs from wild-type Arabidopsis seedlings, wild-type Arabidopsis cells, and fad2 and Fad3⁺ mutant cells. A, Wild-type Arabidopsis seedlings and cells were analyzed independently. Their lipid profiles being similar, the data were combined in the wild-type column. The membranes of fad2 and Fad3⁺Arabidopsis mutant cells are shown as compared to the combined controls. Because very low amounts of DRMs were recovered from the mutant cell lines, we grouped three to four DRM fractions from different experiments to perform lipid analyses. The results are expressed as percent of total lipids (means of two analyses corresponding to 6–8 experiments). Lipid abbreviations are as in the legends of Figures 1 and 2. B, Total fatty acid composition of DRMs isolated from microsomes of Arabidopsis wild-type and fad2 and Fad3⁺ mutants. The results are expressed as percent of total fatty acids (as in A, means correspond to two analyses from 6–8 grouped experiments).