Figure 2.

Purification of NtDES1 expressed in E. coli. Soluble protein extracts correspond to 180 μL of E. coli culture from control (lane 1) or IPTG-induced (lane 2) bacterial cells carrying pGEX-NtDES1. The GST-NtDES1 protein bound to the affinity column was either eluted with 10 mm reduced glutathione (lane 3, 875 ng protein) or digested with factor Xa, leading to the release of NtDES1 in the incubation buffer (lane 4, 210 ng protein). After separation on 10% polyacrylamide gel, proteins were stained with Coomassie Blue (lanes 1 and 2) or silver nitrate (lanes 3 and 4). Position of protein standards are indicated for each gel.