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. 2007 Jan;143(1):326–338. doi: 10.1104/pp.106.086306

Figure 5.

Figure 5.

Analysis of isoflavonoid content and IFS and CHS gene expression in RCAT Angora and Harovinton. A, Total embryo isoflavonoid content in RCAT Angora (RCAT) and Harovinton (Hvtn) during embryo development as determined by HPLC analysis. Data are mean values from three independent experiments. B, Comparison of temporal expression patterns of CHS genes in RCAT Angora (RCAT) and Harovinton (Hvtn) developing embryos at 30, 40, 50, 60, or 70 DAP obtained from microarray analysis. C, RT-PCR analysis of IFS1, IFS2, CHS7, and CHS8 transcripts with gene-specific primers. Template for RT-PCR was total RNA isolated from developing embryos (30–70 DAP) from RCAT Angora (RCAT) and Harovinton (Hvtn). Shown as control is rRNA visualized by staining with ethidium bromide (EtBr).