Table I.
Primer sets used for gene-specific PCR amplifications of EXPA and EXPB sequences of maize
Forward primer positions are given relative to either the start or the stop codon (except when unavailable). GenBank numbers refer to either the full-length cloned sequence when available (underlined) or the TC of EST assembly.
| Gene Name | GenBank No. | Length | Primer Sequence (5′–3′) Forward / Reverse | Position from Start | From Stop |
|---|---|---|---|---|---|
| ZmEXPA1 | AF332169 | 1,090 | CGAGTGTGACTGTGAGCAAGAGA/GCCTTGCAGGTTTTTGTTTTG | +21 | |
| ZmEXPA2 | AF332170 | 1,294 | GCGTTAGCATGGTGTTTTAGTGT/GAGGCCCTAAACGCATAATTG | +181 | |
| ZmEXPA3 | AF332171 | 1,031 | TGGCTCAAGATCGGCATCTA/ACAAGCGAACGAGGCAGAAA | −360 | |
| ZmEXPA4 | AF332172 | 929 | CTACTCACTCCTGCTACCGTACTA/GAATCCCATCCACACATCTT | +95 | |
| ZmEXPA5 | AF332173 | 1,029 | GCCAGTTCTGAGGATGAACAGC/TGGTCCGATCCAGTCCGTAA | −8 | |
| ZmEXPA7 | TC310903 | 1,178 | GACGTTCCGGGTGATGACTA/GTGCATGAGCGAAAGAATGA | −107 | |
| ZmEXPA8 | TC287543 | 1,048 | TGGTCGTTCGTGGACAATAA/GCTGTCCGTGGTGTCAGTTA | +5 | |
| ZmEXPA9 | TC292888 | 1,066 | GAGGCGCCATGGTTTTATGA/CCTACCTCCGTTAATCTGCAAGC | +116 | |
| ZmEXPA10 | TC298364 | 1,226 | TTCTGTAGAGAATGGAGTGCCG/CCGGACATACCCGAATCC | −5 | |
| ZmEXPA11 | TC292885 | 1,349 | GCCGGCGAGGAAGAGCT/GTACACGCCGGGAATGCG | −86 | |
| ZmEXPA12 | TC298359 | 1,317 | GTTGGGCTGTGGTGTACATTCTC/GGAAAACCATTTATGACCAAGAGC | +60 | |
| ZmEXPA13 | TC291600 | 1,159 | CGGCGGTGGTGATGTAAGTAGA/GGAAATCCTTTCGCCATTCAAT | +28 | |
| ZmEXPB1 | AF332174 | 1,080 | CTACACTTCCAACGTCCAATTCTACT/TTCGATCATGAACCCGAACA | −25 | |
| ZmEXPB2 | AF332175 | 907 | CACCACCCACCACTACTACCA/AACGACTCAAAGGACCATGACAA | +30 | |
| ZmEXPB3 | AF332176 | 788 | GTATATGCCATGCGTCGTAC/TATCATCGATCCGATCCGTCC | +5 | |
| ZmEXPB4 | AF332177 | 1,273 | ACCATCGTAATCACCGACCA/CTTCAACCTCCTCTTTCATTCTCT | −474 | |
| ZmEXPB5 | AF332178 | 650 | GCCAACCACAGGCTACAG/CCAAATGACACCGAATCG | −129 | |
| ZmEXPB6 | AF332179 | 1,142 | CTCGCAGGACAGATCCTTGT/GGCACCGTATCTGGTAGCAT | +113 | |
| ZmEXPB7 | AF332180 | 1,173 | GGCCAACTCTGCGTCGTG/GGTCAGCCACCAGCGTC | −190 | |
| ZmEXPB8 | AF332181 | 1,479 | TACAAGCTACGCTCTCCCGC/GAAAAAATGCACTGACGAAAGG | +401 | |
| ZmEXPB10 | AY104999 | 860 | GGTTGTGGATCCTGCTTCGAGAT/GACCCTTCGAAATTGCATGT | −513 | |
| ZmEXPB11 | AY104125 | 1,191 | TCAAGTGCGATAAGCCTGTG/TGCACTTAACCCTTCGGAAC | +319 | −491 |
| ZmEXPB12 | TC287648 | 1,438 | GTGTAAGAAGAGGAGGAGGACA/GAAAAAAGAAACCGACACCGAC | +175 | |
| ZmEXPB13 | TC280521 | 1,720 | GGCTTGATCTGTTTGGTATTGTTG/TGAAACTCTGGACCGACGAC | −51 | |
| ZmEXPB14 | TC280520 | 1,191 | CGTCGTTGTGCTTGATTAGTTTG/GCCTTCCTCCATTTCGTTCC | +40 | |
| ZmEXPB15 | TC311993 | 1,172 | CGCCTGCTATCAGGTGAAAT/CCCGAGTGTACTGGATCTTGA | +248 | |
| ZmEXPB16 | TC289097 | 1,568 | CGTCGTGTTGTTGTTACTGGTGTT/CGATGCGATGCAAGAAAAATAC | −34 | |
| ZmEXPB17 | TC311991 | 650 | TAACGATGGCGGAATAACAGAA/GGTACGGTATAGGCACACGCAC | +0 | |
| ZmEXPB18 | TC310578 | 1,216 | ATGCAGAACAACTCCGGGTA/TACTGGACGAAGGAGCGGTA | −20 | |
| ZmEXPB19 | TC287642 | 1,608 | TGCATGCATCCTGGCTTTT/AAATCAACAGCACGTTCAGACA | +60 | |
| ZmEXPB20 | TC310589 | 809 | ATCCCACAATTCATCTCCTGCA/GATCTACCCGATCGAGCTGAG | +3 | |
| ZmEXPB21 | TC308480 | 888 | ATGTTTGTGGTATTGGCGTTGTG/ACATCACTCCCCCCTCTCAG | −21 | |
| ZmEXPB22 | BG873846 | 543 | ACCAGATACGGTGCCTCAAC/CACCCTCCTGAACTGGATGT | + | − |