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. 2007 Jan;143(1):263–277. doi: 10.1104/pp.106.088609

Figure 1.

Figure 1.

Manganese is required for photosynthetic electron transfer and growth of Chlamydomonas. Cells adapted to Mn deficiency (see “Materials and Methods”) were transferred to TAP medium containing the indicated amounts of supplemental manganese and grown photoheterotrophically. A, The visual phenotype of Mn deficiency is shown after 3 d growth. B, Growth was measured by counting cells every 24 h with a hemocytometer. C, Room temperature chlorophyll fluorescence induction kinetics of cells grown (96 h) at the indicated manganese concentration. D, Restoration of fluorescence induction was monitored as a function of time after addition of 25 μm manganese to a Mn-deficient culture (0 μm). The pattern from a Mn-replete culture is shown for comparison (25 μm). All experiments were performed in experimental duplicate in strain CC425 and verified in strain CC1021.