Figure 2.

Loss of the OEE complex in Mn-deficient cells. A, Thirty micrograms of total membrane protein was loaded onto a denaturing polyacrylamide gel and PSII proteins D1, OEE1, OEE2, and OEE3 (PsbO, P, and Q, respectively) were analyzed for abundance in extracts of cells grown in medium with the indicated manganese concentrations. The α- and β-subunits of CF₁ (chloroplast ATP synthase) are used as a loading control. The experiment shown is representative of three independent experiments. B, Total cell extract from Mn deficient (0 μm) or Mn replete (25 μm) was separated into soluble and pellet fractions (see “Materials and Methods”). The sum of the soluble and pellet fraction equals total cell extract. Samples were analyzed as for part A. The experiment shown is representative of two independent fractionations.