Figure 5.
Relative expression of oxidative stress response genes. RNA was isolated from cells grown in TAP medium containing the indicated manganese supplement plus excess Fe (50 μm). Relative expression was normalized to CβLP expression, and average CT value was calculated from technical triplicates. The error bars represent variation from biological duplicates. Expression was calculated by the 2−ΔΔCT method. All experiments were performed in experimental duplicate and also at two different concentrations of iron (18 and 50 μm).