Table I.
Primers used for real-time PCR
The primer pairs for each gene model (corresponding to the Version 3.0 draft genome) are shown. The upper row of each set corresponds to the forward direction for the gene model and the lower row to the reverse direction. All primer sequences are written 5′ to 3′. The percent efficiency for each primer pair is calculated based on the theoretical doubling of product at each cycle. For 100% efficient primers, the amount of product doubles at every cycle.
| Name | Protein ID | Percent Efficiency | Primer Pair |
|---|---|---|---|
| APX1 | 186597 | 91 | TCAAGGAGATCAAGGCCAAG |
| GCCGCTCAGTCCAGAGTAAC | |||
| CAT1 | 150104 | 86 | CACGAGGGCTTCATGAACTT |
| CTTGGCGATCACCATCTTCT | |||
| COPT1 | 196102 | 106 | TCTGGTTTGCTGGGCTCTCC |
| GTACACCGCCAGTCCCTCGT | |||
| COT1 | 116945 | 101 | CGCCGACCACCACCTGTATC |
| GAGCACGCTGTAGGCAAGCA | |||
| CTP1 | 121438 | 92 | GGTGTTGACCTGGCTACGCTGTG |
| GCCACCGGTGCCAAGGGAGGA | |||
| CTR1 | 196101 | 81 | CCTTCAACATCGGCTTCTTC |
| CCCTTCTTGTGGTCAGCAGT | |||
| CTR2 | 196096 | 87 | CGCTGCTGAACCTCATCTC |
| GCAGGTGGCCCAGGAATAG | |||
| CβLP | 105734 | 108 | GCCACACCGAGTGGGTGTCGTGCG |
| CCTTGCCGCCCGAGGCGCACAGCG | |||
| FEA1 | 129929 | 93 | TGCTGGGCCTGCAGGGCGTGTC |
| ACGGCGGAGGCCTTGAAGTTGC | |||
| FSD1 | 182933 | 95 | CCGTGGTGGAGGGCAAGACCCCCAT |
| TCGCCTGGCGCCGACATGCTTGTGA | |||
| GPX3 | 137012 | 108 | GACAAGGCGGTTCTGGTCGT |
| AGCCCTGCTTGCCGTACTTG | |||
| GPX4 | 188373 | 96 | GCAAGGACCTCATCTGGAAC |
| TATAGATCGCCCCCTCAACA | |||
| GPX5 | 143122 | 87 | CTAGCAACTGCGGCTTTACG |
| GCTTCTTTTGGCTCTTCAGG | |||
| HMA1 | 195998 | 97 | ACGGATGACGAACAGCAGCA |
| CTCACACAGCCCCTTCCACA | |||
| IRT1 | 196644 | 104 | GGGCTCTTCGCACGTCTTCA |
| AGGGGACCAGGAAGCCAAAA | |||
| IRT2 | 174212 | 97 | GGGGCTACTGATGGCGGTGA |
| GTAGATGGGTGCGGCGATGA | |||
| MSD1 | 53941 | 102 | TGATGACCGCGCTGCCGGACGTGGC |
| TCACAGCGGCACGGTGCCGGCCTGG | |||
| MSD2 | 193511 | 101 | ACAAGCTGGAGGTGGTGACGCTGCC |
| TTACTTCTTGGCGGCGGCCAGGCG | |||
| MSD3 | 196645 | 109 | ACCAGACCCACCCAGGAG |
| CAGCCCCAACCAGGATAAC | |||
| MSD4 | 114882 | 88 | CCATGAGTGGGTTGTCCTG |
| GACACTGGAACCACCAGATG | |||
| MSD5 | 115390 | 90 | CACAGCCAACCAGGACAACC |
| CGCGGACCAGTTTATCAG | |||
| MSRA2 | 196103 | 100 | ACCGCGTGCGGCTGGCCACCA |
| GAGCCACGGCGCATCATGGA | |||
| MTP1 | 119301 | 95 | CGTGTGGCTTGAGCGAAGAG |
| GCTTGCGTGCGATGATACGA | |||
| MTP2 | 150298 | 97 | ATGAGTGTGCGGGAGTCGCA |
| GGCAGTGGCTTCATCACGTC | |||
| MTP3 | 195957 | 96 | ATCGAGGCTCTTGACACTGT |
| GTAAGGCGCTGCTCAGCGTC | |||
| MTP4 | 150298 | 101 | ACATGTGTGTGCGGGAGTCG |
| CTTGTGCCGGTGCAGGGACC | |||
| MTP5 | 149470 | 96 | CCTTCATCCTCAGCCATAGCA |
| CGAGAGGTTGGGAAGTGAG | |||
| NIK1 | 173008 | 102 | CCACCTACGCCACAGGCATC |
| TGCCGATCACGAACATGGAG | |||
| NRAMP1 | 128400 | 109 | GCGCCGCATTATCACCCGTGGCGCCGCCATC |
| CAGCGGTTGGCGAAGTTGCCGAGGTAC | |||
| NRAMP2 | 113049 | 87 | GCGACGCCTACGAGCTGCTGGCCACCTC |
| ACTTGATGGCCAGAAAGCCCTCC | |||
| ZIP1 | 122719 | 100 | GCTGGCTTCTACAGCCTCACC |
| ACGAACCTGCCCATGTCCTC | |||
| ZIP3 | 100805 | 92 | CCGTCATCTCGCTGTTCGTG |
| CCCTTTGGGTCCTGTTCGTG | |||
| ZIP6 | 183171 | 86 | GGCAAGAACCGGCTGCTAAA |
| CTCCTGCGATGGGTGTTCCT | |||
| ZRT1 | 146152 | 90 | TCGTCTTCGGGTTCCTCCTG |
| CGGGGGTGTTATCCTCGTTG | |||
| ZRT2 | 107206 | 97 | GGCTGCGTGTTCCACTCCTT |
| CTGATGCCCTCCGCAAACTG | |||
| ZRT3 | 168584 | 96 | CCTTTCCCACAAACACGCAAG |
| TGCCGATGATGATGCTGTGA | |||
| ZRT4 | 196642 | 92 | GGGCTTCCTGTGGTCTGTGG |
| GCCGTACATGAGGGGGTCTG | |||
| ZRT5 | 196756 | 100 | TGCGTGTTCCACAGCTTCATC |
| ACCACAGACGCCAGCGAGAC |