Table II.
Fe-responsive gene expression during Mn deficiency
Gene expression was assessed by real-time PCR. Cells were made Fe or Mn deficient and harvested as described in “Materials and Methods.” Relative expression was normalized to CβLP expression, and average CT value was calculated from technical triplicates and compared to expression in replete conditions. Expression was calculated by the 2−ΔΔCT method (Livak and Schmittgen, 2001).
| Gene | Relative Gene Expression
|
|
|---|---|---|
| 1 μm Iron | 0 μm Supplemental Mn2+ | |
| FEA1 | 2.0 × 102 | 2.4 × 101 |
| FOX1 | 3.5 × 101 | 4.1 × 100 |
| FRE1 | 3.3 × 102 | 1.9 × 102 |
| FTR1 | 5.3 × 101 | 4.7 × 100 |