Temporal and spatial root AtAmt1.1 expression as nitrogen starvation progresses from 2 d poststarvation (A, C, D) to 5 to 7 d poststarvation (B, E, F). A and B, LUC expression is depicted in red. GUS images of root hairs (C) and newly emerging lateral roots from the primary roots (D). E and F, GFP and GUS images, respectively, showing expression in the entire root system, including the steles of primary and lateral roots. Lengths are indicated with bars (in millimeters). All images were acquired at the midpoint of the light period.