Skip to main content
. 2007 Jan;143(1):28–37. doi: 10.1104/pp.106.092643

Figure 1.

Figure 1.

Guard cells in intact N. tabacum plants impaled with triple-barreled electrodes. A, Close up of an N. tabacum leaf impaled with triple-barreled electrode (left), guard cells in the abaxial epidermis were visualized with a water emersion objective (middle). Experimental solutions were perfused through a hypodermic needle (left back) over the cuticle to a suction pipette (right). B, Overview of the experimental setup with the upright microscope (middle), microelectrode amplifiers (left), and the N. tabacum plant (right). C, N. tabacum guard cell impaled with a triple-barreled electrode, through which FURA2 was injected, located with the tip in the cytoplasm. D, Guard cell injected with FURA2 through a triple-barreled electrode with the tip in the vacuole. E, Guard cell injected with FURA2 in the cytoplasm, but in which the FURA slowly appeared in the vacuole. F, Same guard cell as in E but 15 min later.