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Radio-HPLC analysis of the biosynthetic product (retention time Rt = 21.9 ± 0.1 min) generated from 2-debenzoyl-7,13-diacetylbaccatin III and [7-14C]benzoyl-CoA by the recombinant benzoyltransferase. The Upper trace shows the UV profile (A228) and the Lower trace shows the radioactivity profile (in mV), both of which coincide exactly with the retention time of authentic 7,13-diacetylbaccatin III.
