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Coupled reverse-phase HPLC APCI-MS analysis of the biosynthetic product (Rt = 43.6 ± 0.1 min) generated by the recombinant benzoyltransferase with 2-debenzoyl-7,13-diacetylbaccatin III and benzoyl-CoA as cosubstrates (Upper) and of authentic 7,13-diacetylbaccatin III (Rt = 43.6 ± 0.1 min) (Lower). The diagnostic ions are at m/z 688 (P + NH4+), 671 (PH+), 652 (P⋅+ − H2O), 611 (PH+ − CH3COOH), 593 (m/z 652 − CH3COO), 551 (m/z 611 − CH3COOH), and 489 (m/z 611 − PhCOOH).
