TABLE TWO.
Identification of myosin XVIIIA and cellular myosin IIA in mouse SP-R210 Mouse SP-R210 was purified from mAM cells by affinity chromatography and size exclusion as described under “Experimental Procedures.” The excised silver-stained SP-R210 band was in-gel trypsin-digested, and the resulting peptides were analyzed by nanoflow HPLC interfaced to electrospray ionization on a quadrupole ion trap mass spectrometer (52). Sequenced SP-R210 peptides are aligned to the corresponding mouse (Ms) and human (Hm) MyoXVIIIA and MyoIIA peptides. Underlined boldface letters are single amino acid substitutions in the corresponding HmMyoIIA sequence.
| A) Myo XVIIIA | ||
| Peptide 1 AGSATVLSGSIAGLEGGSQLALR | Peptide 2 | ELQTQYDALKK |
| MsMyoXVIIIA 973RAGSATVLSGSIAGLEGGSQLALR996 | MsMyoXVIIIA | 1323KELQTQYDALKK1334 |
| HuMyoXVIIIA977RAGSATVLSGSIAGLEGGSQLALR1000 | HuMyoXVIIIA | 1327KELQTQYDALKK1338 |
| B) Nonmuscle myosin IIA | ||
| Peptide 3 EDQSILCTGESGAGK | Peptide 4 | QTLENERGELANEVK |
| MsMYOIIA 165REDQSILCTGESGAGK180 | MsMYOIIA | 1219KQTLENERGELANEVK1234 |
| HuMYOIIA 165REDQSILCTGESGAGK180 | HuMYOIIA | 1219KQTLENERGELANEVK1234 |
| Peptide 5 QLLQANPILEAFGNAK | Peptide 6 | LQVELDSVTGLLSQSDSK |
| MsMYOIIA 209RQLLQANPILEAFGNAK225 | MsMYOIIA | 1277KLQVELDSVTGLLSQSDSK1295 |
| HuMYOIIA 209RQLLQANPILEAFGNAK225 | HuMYOIIA | 1277KLQVELDNVTGLLSQSDSK1295 |
| Peptide 7 VIASGVLQGNIAFK | Peptide 8 | DFSALESQLQDTQELLQEENR |
| MsMYOIIA 341RVISGVLQLGNIAFK355 | MsMYOIIA | 1301KDFSALESQLQDTQELLQEENR1322 |
| HuMYOIIA 341RVI SGVLQ LGNIVFK355 | HuMYOIIA | 1301KDFSALESQLQDTQELLQEENR1322 |
| Peptide 9 VVSAVLQLGNIAFK | Peptide 10 | ALEQQVEEMK |
| MsMYOIIA 341RVISGVLQLGNIAFK355 | MsMYOIIA | 1529RALEQQVEEMK1539 |
| HuMYOIIA 341RVISGVLQLGNIVFK355 | HuMYOIIA | 1529RALEQQVEEMK1539 |
| Peptide 11 VVFQEFR | Peptide 12 | LEVNLQAMK |
| MsMYOIIA 711RVVFQEFR718 | MsMYOIIA | 1557RLEVNLQAMKA1566 |
| HuMYOIIA 711RVVFQEFR718 | HuMYOIIA | 1557RLEVNLQAMKA1566 |
| Peptide 13 ALELDSNLYR | Peptide 14 | DLEAHIDTANK |
| MsMYOIIA 745KALELDSNLYR755 | MsMYOIIA | 1620KDLEAHIDTANK1631 |
| HuMYOIIA 745KALELDSNLYR755 | HuMYOIIA | 1620KDLEAHIDSANK1631 |
| Peptide 15 LMEMET LQSQLMAEK | Peptide 16 | QLEEAEEEAQR |
| MsMYOIIA 867RLTEMETMQSQLMAEK882 | MsMYOIIA | 1877RQLEEAEEEAQR1888 |
| HuMYOIIA 867RLTEMETLQSQLMAEK882 | HuMYOIIA | 1877RQLEEAEEEAQR1888 |
| Peptide 17 ELEDATETADAMNR | Peptide 18 | IIGLDQVAGMSETALPGAFK |
| MsMYOIIA 1898RELEDATETADAMNR1912 | MsMYOIIA | 617RIIGLDQVAGMSETALPGAFK637 |
| HuMYOIIA 1898RELEDATETADAMNR1912 | HuMYOIIA | 617RIIGLDQVAGMSETALPGAFK637 |