FIGURE 4.

Bacterial expression of the reconstructed cTnT fragment. 5’-truncated mouse cTnT cDNA was constructed according to the NH₂-terminal truncation site for protein expression in E. coli (see Materials and Methods). S/D and Tφ in the pAED4 expression vector indicate the Shine-Dalgarno ribosomal binding site and the transcription termination sequence, respectively. The cTnT fragment expressed from the truncated cDNA shows a size identical to that of the cTnT fragment produced in ischemia-reperfused cardiac muscle (the slightly slower gel mobility seen in the blot may be due to the addition of an NH₂-terminal Met in the expression construct), indicating that the NH₂-terminal truncation is the only primary structure modification.