Table 2.
Primers and characteristics of microsatellites loci.
| Locus | Motif | Origin | Function | Primer sequences (5'-3') | Temp | Linkage group | Genebank Accession |
| FMT 07 | [GA]28 | GL | Non coding | GCAGAGAAAAGTAACACCATC CAGGTTCTTTCTTACTTTCCC |
60 | 7 | AY294632 |
| FMT 08 | [CT]16 [CA]7 | GL | Non coding | CAATCACTGGAAGCAAGGT AGCCTGCTCATTTGTATTGC |
55 | 7 | AY294633 |
| FMT 11 | [GA]16 | GL | Non coding | GGCCCAACCACAATTTC CATAACTTCCAATAACTGCCA |
55 | 1 | AY294635 |
| FMT 50 | [GA]10 | GL | Non coding | TAGGGACTTGAGGGGGTG CAATATCGTAACACATGGCTC |
60 | 3 | AY294640 |
| FMT 90 | [GA]12 | GL | Non coding | GGATGATGAAGACAATGTTAGCA AGAAGTTATAGAGGGACGAAATTAG |
60 | 1 | AY294642 |
| FMTBN1 | [TA]6 | GB | Non coding | TGTCAATTTCAGTGTTTGCT AGTAAATGACAATAAGGAGT |
50 | 5 | AC126010 |
| MAL367466 | [TTC]8 | GB | Unknown | ACACAAACAAACATTCTCAC CATCGGTTGTTAGAGAGAG |
60 | 2 | AL367466 |
| ENPB1 | [AC]6 | GB | ENBP 1 gene | CACTTCCCACTGTCCTAGTCCTAC GACTCGTCATCACCAGTTTCATCC |
55 | 1 | AJ002479 |
| MTIC37 | [AAG]10 | GB | Unknown | AAGAGCAAGCAAGAGGATGC TCTTGGCCATTACAATATCATCA |
55 | 4 | AL373844 |
| MTIC59 | [AAG]6 | GB | Unknown | CAAATTTGGAGAAGGCGAAG CGGTCTCTTCATATTCGCTGA |
55 | 5 | AL377524 |
| MTIC126 | [AC]8 | GB | Unknown | CCCAGTTGCAGACTCTCTCA GAAGGGTTTACCGGAGGAAG |
50 | 6 | AL368250 |
| MTIC243 | [TTG]6 | GB | Unknown | GGAGGAGGTTATAGGTTTGG TCAGTGCTCAGCATCTATGT |
50 | 6 | n.a. |
| MTPG85C | [TC]11 [AC]8 | GL | Non coding | CCAACACAGTCACCTAACCAA GTTTCTCCATTGTTTCGTAGG |
50 | ? | AF274878 |
Microsatellite motif was determined based on the sequence of a cloned allele. Microsatellite loci were obtained from microsatellite-enriched genomic libraries (GL) or using sequence information retrieved from GenBank (GB).
Temp: optimal annealing temperature for the PCR amplification
n.a. non available