Figure 6.
The P. pastoris Atg9 orthologue also localizes to mitochondria. (A) PpAtg9 is partially distributed to mitochondria. The PpATG9-GFP (FRPPY002) strain was grown to early log phase or starved in SD-N medium for 1 h, incubated in the presence of 0.05 μM MitoTracker Red CMXRos for 30 min and fixed as described in Materials and Methods before imaging. (B) PpAtg9 does not localize to the ER. The DsRedHDEL PpATG9-GFP (FRPPY003) strain was grown, fixed and imaged as in (A).