Figure 8.

U-II-mediated inositol phosphates accumulation: Inositol phosphates accumulation in response to human and goby U-II (1 pM – 1 μM) in HEK-293 cells stably transfected with mouse (a) or monkey (b) UT receptor. The cells were treated with myo-[³H]inositol (1.0 μCi ml⁻¹) overnight and then washed to remove excess myo-[³H]inositol. The cells were challenged with different concentrations of human or goby U-II for 5 min, and the inositol phosphates were separated from free inositol using ion-exchange chromatography. Data are the mean±s.e.mean values from three different experiments for the accumulation of inositol trisphosphate. Similar observation was made for inositol mono and diphosphates.