Figure 6.

Effects of SNP and 8-Br-cyclic GMP on [³H]-choline release evoked by electrical field stimulation (EFS) of rat pancreatic segments. The bar charts show the area under the curve (AUC, see Figure 5) for [³H]-choline release from preloaded segments during (A) 10 min pre-stimulation in the absence (KH solution alone) and presence of either SNP (1×10⁻³ M) or 8-Br-cyclic GMP (1×10⁻⁴ M) and (B) combined 10 min EFS stimulation (20 Hz, 50 V, 1 ms) plus post-stimulation (20 min) in the absence and presence of either SNP (1×10⁻³ M) or 8-Br-cyclic GMP (1×10⁻⁴ M). Data were obtained by substracting the averaged basal values (first 0 – 10 min) from either the pre-stimulation values (10 – 20 min) or the combined stimulated (20 – 30 min) plus post-stimulated (30 – 50 min) values. Each point is mean±s.e.mean, n=6. P<0.05 for (d) compared with (e) and (f), but (a), (b) and (c) are not significantly different from one another.