Table 4.
Neutralization Titers of Guinea Pigs Immunized with HIV-1 Subtype B Monomeric gp120 Immunogens
|
B. 6101 gp120 |
B.BaL gp120 |
|||||||
|---|---|---|---|---|---|---|---|---|
|
Guinea Pig Number |
Guinea Pig Number |
|||||||
| HIV-1 Isolate | 858 | 859 | 860 | 861 | 889 | 890 | 891 | 902 |
| B.BX08# | 21 | 20 | <20 | 24 | 368 | 540 | 637 | <20 |
| B.QH0692.42 | <20 | <20 | <20 | <20 | 27 | 38 | 55 | 45 |
| B.QH0515.1 | <20 | <20 | <20 | <20 | <20 | 36 | <20 | 40 |
| B.BaL.26 | 107 | 166 | 201 | 540 | 112 | 1,844 | 1,475 | 507 |
| B.SS1196.1 | 141 | 165 | 210 | 577 | 105 | 2,395 | 2,475 | 1,475 |
| B.SF162.LS | 265 | 180 | 227 | 1,298 | 530 | 130 | 1,397 | 424 |
| B.JRFL-MC ** | <20 | <20 | <20 | <20 | <20 | 23 | 43 | <20 |
| B.BG1168.1 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
| B.3988.25 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
| B.6101.10 | <20 | <20 | <20 | <20 | <20 | <20 | <20 | <20 |
50% Neutralization titers of serum after 4th or 5th immunizations as determined in the pseudotyped HIV-1 neutralization assays as described in Methods. Neutralization was considered positive (number in bold) if the titer of post-immune serum minus the titer of pre-immune bleed serum was >30. Guinea pigs anti-sera were tested as negative against 11 subtype C pseudotyped viruses including TV-1.21, DU123.6, DU172.17, 02ZM233.PB6, 92BR025.9, 02ZM197.PB7, 96ZM651.2, DU151.2, 97ZA012.29, DU422.01 and DU156.12, and 2 subtype A pseudotyped viruses, 92RWO20.2 and 92UG037.02. ND = Not done.
Assayed in Luc M7 cells using virus stock grown in PBMC.
A full-length molecular clone, and the assay stock of virus was produced by transfection in 293T cells.