Skip to main content
. Author manuscript; available in PMC: 2007 Feb 1.
Published in final edited form as: Neurobiol Dis. 2006 Sep 28;25(1):17–26. doi: 10.1016/j.nbd.2006.08.004

Figure 3. HIV gp120 up-regulates E2F1 pro-apoptotic targets in rat neurons.

Figure 3

Treatment with gp120IIIB (200 pM) increased transcriptional activity in rat primary neurons as determined by gene reporter assays (A, mean ± SEM of one representative experiment; *p<0.01 vs control). E2F1 over-expression was used as positive control for the assay. Elevated levels of transcriptional targets of E2F1 (Puma and Cdc2) were also found in neuronal extracts from primary rat cultures treated with gp120 (B–C) as well as in neurons transfected with the E2F1 expression plasmid (D). The effect of gp120IIIB was blocked by pre-treatment of cultures with AMD3100 (100 ng/ml) 15 min before the addition of the gp120.