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. 2006 Dec 27;1(1):e131. doi: 10.1371/journal.pone.0000131

Figure 3. The only essential role of Rok during cytokinesis is to maintain phosphorylation of myosin II RLC.

Figure 3

(A) S2 cells expressing RLCT20S21-GFP or RLCE20E21-GFP were treated with dsRNAs for 5 days to deplete Rho1, Rok, or Citron and then fixed and stained with DAPI. The fraction of binucleated cells (mean, ±SE for approximately 200 cells) was determined by fluorescence microscopy. Expression of RLCE20E21-GFP rescues depletion of Rok, but not Rho1 or Citron. (B) Expression of phospho-mimic RLC rescues Rok depletion. S2 cells expressing RLC-GFP chimeras (green) and RFP-α-tubulin (red) were depleted of the Rok by RNAi treatment for five days, and timelapse fluorescence microscopy was used to film cells as they attempted to divide. RLCT20S21-GFP (Row 1) was not recruited to the equatorial cortex at anaphase and the cells did not contract. RLCE20E21-GFP (Row 2) was recruited to the equatorial cortex during mitosis and the cells contracted. The scale bar = 5 µm.