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. 2006 Dec 20;1(1):e55. doi: 10.1371/journal.pone.0000055

Figure 3. Spectratyping and T-array for Jurkat T-cell clone mixed with peripheral blood CD4+ T-cells.

Figure 3

Jurkat cells were added in different dilutions to a background of peripheral blood CD4+ T-cells.

(panel A) CDR3 spectratyping with Vβ12-specific primer.

The arrow indicates a length identical to 14 amino acids, which is the length of the Jurkat CDR3β.

(panel B) A detail of the T-array scans.

White arrows indicate hexamer sequence GTTCGG, which is complementary to the first six nucleotides Jurkat NDNβ region.

(panel C) Signal intensities of all 4096 spots of the T-array.

Black arrows indicate hexamer sequence GTTCGG.