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. 2006 Dec 20;1(1):e55. doi: 10.1371/journal.pone.0000055

Figure 5. T-array analysis of CMV-specific cells in vivo.

Figure 5

(A) Peripheral blood was drawn 9 weeks after primary CMV infection of a CMV-seronegative renal transplant recipient, and sorted for CMV+ IFNγ-producing T-cells.

(B) cDNA was amplified using anchored PCR (ref. 20).

(C) Vβ's were analyzed by spectratyping and 11 Vβ-families were cloned and sequenced (ref. 18).

(D) The most prominent Vβ/Jβ combination as identified by (C), i.e. the Vβ6.1/Jβ2.7+pool, was amplified by Vβ6.1-and Jβ2.7-primers and loaded on a hexamer array.

(E) CDR3 regions, clonal frequencies, and joining sequences of the identified Vβ6.1/Jβ2.7+T-cell clones.

(F) Vβ6.1/Jβ2.7-specific T-array with the annealer CTACGAGCAGTACTTCGGG, which matches the germline Jβ-2-7 sequence with 3 nucleotides deleted.