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. 2006 Dec 20;1(1):e95. doi: 10.1371/journal.pone.0000095

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Pourmand et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A representative pyrogram illustration. Comparison of pyrosequencing results obtained using the de novo (top) and consensus pre-programmed (bottom) sequencing methods for site 3 (a clade marker) in sample A1 (goose/Guangdong/1/96). Both methods give the sequence GACAAAGCTCTATCAAAAC for our laboratory stock. In contrast the GenBank sequence (accession # AF144305) reads GACAAAGCTATATCAAAAC. Database information was found to be based off wild-type sequence information, while the sample we analyzed was obtained via reverse genetics. A longer read length is obtained via the consensus pre-programmed dispensation method (22 versus 12 nucleotides).