Figure 2. Development of anticipatory increase in locomotor activity prior to food administration in wildtype (A) and ACHE mutants (B) of different age, and in IR zebrafish (C).

X-axis: % change in activity level within 30 min prior to food administration, as compared to preceding 90-min period.

First day of significant (p<.05) change in group behavior in young (*) and middle-aged (**) fish.

Note that BL was assessed after 7 days of new timing of daily food administration in white/white environment.

Young fish – diamond, middle-aged fish – square, old fish – triangle.

Wildtype – black; ACHE mutants – green; IR zebrafish – orange.