Figure 2.

cno² zygotic null mutants display gain and loss of progenitors.

All panels show lateral views of stage 11 embryos stained with an α-Eve antibody.

(A) Eve wildtype (wt) expression in a subset of dorsal muscle/heart progenitors and founder cells (2–3 cells) per hemisegment.

(B) cno² mutants show gain (arrow), loss (arrowhead) or a wildtype number (asterisk) of Eve⁺ progenitors in different hemisegments.

(C) Embryos in which N^Act, Wg and Ras^Act are simultaneously expressed in the mesoderm display a cno²-like phenotype: gain (arrow), loss (arrowhead) and wildtype number of Eve⁺ progenitors (asterisk).

(D) Overexpression of Ras1^Act in the mesoderm induces the specification of extra Eve⁺ progenitors.

(E) Overexpression of N^Act in the mesoderm inhibits Eve⁺ progenitor specification.

(F) Embryos in which Wg is overexpressed in the mesoderm show gain of Eve⁺ progenitors in intersegmental regions (arrow).

(G) Table shows % of embryos of the indicated genotype that display a wildtype number of Eve⁺ progenitors, Eve⁺ progenitors gain, loss and both (gain and loss).

cno² and cno^mis1 are null and hypomorph mutant alleles, respectively; Df(3R)^6-7 removes cno. cno gof phenotype (UAS-cno) is also variable.

Legends for bars are shown below the table.

At least 70 embryos of each genotype were counted. P<0.0001 for all genotypes except for cno^mis1 (P = 0.0017).