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. 2006 Dec 20;1(1):e26. doi: 10.1371/journal.pone.0000026

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Expression level of Tcl1 gene effects on cell proliferation.

The number of ES cells decreased when Tcl1 was knocked down by shRNA.

(B) RT-PCR and Western blot analysis of ES cells with shRNA of Tcl1 gene.

Tcl1 gene expression affected active Akt1 (p-Ser.473 Akt1), but not wild type of Akt1.

(C) Reversibility of Tcl1 downregulation effect.

The number of cells was rescued in the absence of Tet.

(D) Photomicrographs of ES cell cultures.

The number of ES cells decreased when Tcl1 was knocked down by shRNA.

Cell proliferation was rescued in the absence of Tet.

(E) Overexpression of Tcl1 does not rescue cell proliferation while Oct3/4 is repressed.

(F) A model for the involvement of Oct3/4-Tcl1-Akt in ES cell proliferation.