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. 2006 Dec;169(6):2031–2041. doi: 10.2353/ajpath.2006.060146

Figure 4.

Figure 4

Effect of TNF-α on EGF-R expression in gallbladder epithelial cells. Primary cultures of human gallbladder epithelial cells were incubated with or without TNF-α (20 or 40 ng/ml) for 24 hours and were then submitted to Western blot or real-time RT-PCR analyses. A: Western blot analysis of EGF-R examining the effect of TNF-α concentration on EGF-R expression. A representative Western blot of EGF-R and β-actin is shown. In quantitative analysis, results are expressed as the ratio of EGF-R/β-actin signals and relative to untreated cells. They represent the mean ± SEM of three preparations; *P < 0.05 versus untreated cells. B: Real-time RT-PCR analysis of EGF-R gene expression in response to stimulation with TNF-α (40 ng/ml). Results are expressed as mRNA levels normalized to 18S rRNA and relative to untreated cells. They represent the mean ± SEM of four experiments performed in duplicate; *P < 0.05 versus untreated cells.