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. 2006 Dec;169(6):2042–2053. doi: 10.2353/ajpath.2006.060081

Figure 5.

Figure 5

Resistin stimulates MCP-1 expression in HSCs. A: Serum-starved HSCs were exposed for 24 or 48 hours to 500 ng/ml resistin, as indicated. At the end of incubation, conditioned medium was collected, and MCP-1 concentrations were measured by ELISA. *P ≤ 0.05 versus control. Data are expressed as mean ± SE of three independent experiments. B: Serum-starved HSCs were stimulated with 250 ng/ml resistin for the indicated time points. Total RNA was analyzed for MCP-1 gene expression by RNase protection assay, as described in Materials and Methods. Transfer RNA (tRNA) and peripheral blood mononuclear cell RNA (PBMC) were used as negative and positive controls for hybridization, respectively. C: HSCs were incubated in the presence or absence of 500 ng/ml resistin for 48 hours. Aliquots of cell supernatant were analyzed in chemotactic assays using human peripheral blood mononuclear cells, as described in Materials and Methods. The number of cells migrating to the underside of the filters was quantified in 10 high-power fields (HPFs). Results are the mean ± SE of four different experiments.