Figure 6.

Resistin activates different intracellular signaling pathways in HSCs. A: Serum-deprived HSCs were exposed to 500 ng/ml resistin for different time points, as indicated. Ten μg of total cell lysate were separated by SDS-PAGE and sequentially immunoblotted with antibodies specifically recognizing the phosphorylated form of ERK (top) or total ERK (bottom). B: Serum-deprived HSCs were incubated with 500 ng/ml resistin at the indicated time points. Total cell lysates were separated by SDS-PAGE and analyzed by immunoblotting using antibodies specifically recognizing phosphorylated IκBα (top) or β-actin (bottom). A lysate from HepG2 cells exposed to 200 ng/ml TNF-α for 15 minutes was used as positive control (PC). C: The experiment was conducted exactly as described in B, but the membranes were blotted with antibodies against phosphorylated p65^NF-κB (top) or β-actin (bottom). Migration of the molecular weight markers is shown on the left.