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. 2006 Dec;169(6):2042–2053. doi: 10.2353/ajpath.2006.060081

Figure 8.

Figure 8

NF-κB activation by resistin requires intracellular calcium. A: Serum-deprived HSCs were preincubated with 10 μmol/L BAPTA-AM or vehicle for 30 minutes, before the addition of 250 ng/ml resistin for 15 minutes, as indicated. Total cell lysates were separated by SDS-PAGE and analyzed by immunoblotting using antibodies specifically recognizing the phosphorylated form of IκBα. Total protein lysate from HepG2 cells exposed to 200 ng/ml TNF-α for 15 minutes was used as positive control. The membrane was stripped and reblotted with β-actin antibody to ensure equal loading. Migration of the molecular weight marker is shown on the left. B: Serum-starved HSCs were preincubated with BAPTA-AM for 10 minutes and then exposed to 250 ng/ml resistin. NF-κB-dependent gene expression was assessed by luciferase reporter gene assay as described in Materials and Methods. Results are shown as fold increase with respect to untreated cells. *P < 0.05 versus resistin alone.