Figure 4.

A: NOD.Rag1^−/− mice irradiated with 650 rads received 10⁷ activated BDC T cells and were followed for diabetes development. Although control unirradiated NOD.Rag^−/− mice developed diabetes in 5 days (n = 4), irradiated mice showed a delayed onset of diabetes until day 19 after cell transfer (n = 8). B: H&E staining of an islet from an irradiated mouse at day 5 after cell transfer. Note the lack of insulitis as evidenced by few leukocytes around the islet. C: H&E staining of an islet from an irradiated recipient mouse that became diabetic at day 19 after BDC T-cell transfer. Islet shows severe infiltration with loss of β-cell mass. Scale bar = 20 μm.