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Cytotoxicity assay of chromium-labeled β cells in the presence of enriched phagocytes from acute diabetic pancreata. CD11b+- and CD11c+-enriched phagocytes were co-cultured with the chromium-labeled β cells at an effector/target ratio of 50:1 (5 × 105:1 × 104). Chromium release was measured at 0, 4, and 12 hours of incubation, and lysis percentage was calculated.
